We have developed two generations of hardware to perform Bioluminescent Assays in Real-Time, which are now being used to develop assays and demonstrate the performance capabilities of BART. Whilst the underlying amplification and measurement principles are the same, there are a number of bespoke differences in their use:
Lumora has developed a highly portable instrument, likely the simplest ever for molecular amplification and detection, which is straight-forward to manufacture and easy to operate. Underneath its casing the PDQ platform consists of a heater block for isothermal reactions and an array of "off-the-shelf" photodiodes for real-time bioluminescent reading, making it incredibly robust yet meaning cost of goods is remarkably low. An algorithm for data interpretation is built to provide easy-to-interprate results for users, whilst sample data can be inputted & outputted quickly using the full colour touch screen and the built in SD card reader.
We have also developed a laboratory system capable of imaging high density arrays of simultaneous reactions, using the popular microwell plate format. These instruments are routinely used for screening and down-selection of primer designs for inclusivity, exclusivity and general performance characteristics. They can be made available to licensees and other research and development collaborators for external development activities.
In addition, this digital camera based system has also been used with 1534 well plates in proof-of-concept studies for Digital BART